биоинженерии
лаборатория
заведующий кафедрой
Направления
деятельности
02
Генная инженерия и репрограммирование стволовых клеток
01
Редактирование генома с применением CRISPR/Cas9
Компетенции
Культивирование клеток человека и млекопитающих, включая стволовые плюрипотентные клетки
Иммуноцитохимия, флуоресцентная микроскопия, проточная цитометрия и сортировка клеток
ПЦР-анализ, включая количественную оценку экспрессии генов
Транскриптомика (bulk RNA-seq и single-cell RNA-seq)
Сборка генно-инженерных конструкций, методы трансфекции и доставки в клетки
Работа с CRISPR/Cas9: нокаут, нокдаун, активация генов
проекты
Оптимизация средств доставки рибонуклеопротеиновых комплексов CRISPR/Cas9 для нокаута гена CCR5 в гемопоэтических клетках человека с целью лечения ВИЧ-инфекций
Инициативная тема
ИБГ РАН
РНИМУ им.Н.И.Пирогова Минздрава РФ
коллаборации
НЦПЗ Миннауки РФ
ФЦМН ФМБА России
ИБР им.Н.К.Кольцова РАН
ИМБ им.В.А.Энгельгардта РАН
Публикации
Human pancreatic islet-derived stromal cells reveal combined features of mesenchymal stromal cells and pancreatic stellate cells
Ebrahim N, Kondratyev N, Artyuhov A, Timofeev A, Gurskaya N, Andrianov A, Izrailov R, Volchkov E, Dyuzheva T, Kopantseva E, Kiseleva E, Golimbet V, Dashinimaev E. Human pancreatic islet-derived stromal cells reveal combined features of mesenchymal stromal cells and pancreatic stellate cells. Stem Cell Res Ther. 2024 Oct 8;15(1):351. doi: 10.1186/s13287-024-03963-2. PMID: 39380125; PMCID: PMC11463112.
Different iPSC-derived neural stem cells shows various spectrums of spontaneous differentiation during long term cultivation
Galiakberova AA, Brovkina OI, Kondratyev NV, Artyuhov AS, Momotyuk ED, Kulmukhametova ON, Lagunin AA, Shilov BV, Zadorozhny AD, Zakharov IS, Okorokova LS, Golimbet VE, Dashinimaev EB. Different iPSC-derived neural stem cells shows various spectrums of spontaneous differentiation during long term cultivation. Front Mol Neurosci. 2023 May 2;16:1037902. doi: 10.3389/fnmol.2023.1037902. PMID: 37201156; PMCID: PMC10186475.
Tumor Suppressor Properties of Small C-Terminal Domain Phosphatases in Clear Cell Renal Cell Carcinoma
Krasnov GS, Puzanov GA, Dashinimaev EB, Vishnyakova KS, Kondratieva TT, Chegodaev YS, Postnov AY, Senchenko VN, Yegorov YE. Tumor Suppressor Properties of Small C-Terminal Domain Phosphatases in Clear Cell Renal Cell Carcinoma. Int J Mol Sci. 2023 Aug 19;24(16):12986. doi: 10.3390/ijms241612986. PMID: 37629167; PMCID: PMC10455398.
Strategies for HIV-1 suppression through key genes and cell therapy
Sorokina A, Anchakova E, Dashinimaev E. Strategies for HIV-1 suppression through key genes and cell therapy. Front Med (Lausanne). 2023 Nov 29;10:1259995. doi: 10.3389/fmed.2023.1259995. PMID: 38093984; PMCID: PMC10716291.
IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals
Galiakberova AA, Surin AM, Bakaeva ZV, Sharipov RR, Zhang D, Dorovskoy DA, Shakirova KM, Fisenko AP, Dashinimaev EB. IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Neurochem Res. 2022 Apr;47(4):952-966. doi: 10.1007/s11064-021-03497-6. Epub 2021 Dec 2. PMID: 34855047; PMCID: PMC8891101.
The Efficiency of Gene Activation Using CRISPR/dCas9-Based Transactivation Systems Depends on the System Run Time
Artyuhov AS, Dorovskiy DA, Sorokina AV, Shakirova KM, Momotyuk ED, Dashinimaev EB. [The Efficiency of Gene Activation Using CRISPR/dCas9-Based Transactivation Systems Depends on the System Run Time]. Mol Biol (Mosk). 2022 Nov-Dec;56(6):1014-1022. Russian. doi: 10.31857/S0026898422060052. PMID: 36475485.
PDX1 is the cornerstone of pancreatic β-cell functions and identity
Ebrahim N, Shakirova K, Dashinimaev E. PDX1 is the cornerstone of pancreatic β-cell functions and identity. Front Mol Biosci. 2022 Dec 15;9:1091757. doi: 10.3389/fmolb.2022.1091757. PMID: 36589234; PMCID: PMC9798421.
Detection of CCR5Δ32 Mutant Alleles in Heterogeneous Cell Mixtures Using Droplet Digital PCR
Sorokina A, Artyuhov A, Goltsova A, Dashinimaev E. Detection of CCR5Δ32 Mutant Alleles in Heterogeneous Cell Mixtures Using Droplet Digital PCR. Front Mol Biosci. 2022 Feb 21;9:805931. doi: 10.3389/fmolb.2022.805931. PMID: 35265670; PMCID: PMC8898955
Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems
Shakirova KM, Ovchinnikova VY, Dashinimaev EB. Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems. Front Bioeng Biotechnol. 2020 Jul 28;8:882. doi: 10.3389/fbioe.2020.00882. PMID: 32850737; PMCID: PMC7399070.
Neural Stem Cells and Methods for Their Generation From Induced Pluripotent Stem Cells in vitro
Galiakberova AA, Dashinimaev EB. Neural Stem Cells and Methods for Their Generation From Induced Pluripotent Stem Cells in vitro. Front Cell Dev Biol. 2020 Oct 8;8:815. doi: 10.3389/fcell.2020.00815. PMID: 33117792; PMCID: PMC7578226.